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References

 

filebox.vt.edu/users/chagedor/biol_4684/Methods/restriction.html    

http://amrita.vlab.co.in/?sub=3&brch=186&sim=781&cnt=1

http://amrita.vlab.co.in/?sub=3&brch=77&sim=694&cnt=1

http://biotechlearn.org.nz/themes/bacteria_in_biotech/how_to_add_foreign_dna_to_bacteria

http://books.google.com.my/books?id=Gv4j859JpjwC&pg=PA171&lpg=PA171&dq=linkers+and+adaptors+in+molecular+biology&source=bl&ots=RoRgYlOVGr&sig=NBCQDYqzmFgxnd5MO4brreGrJu0&hl=en&sa=X&ei=guKFVKGzBoOSuQSik4H4Bg&ved=0CBsQ6AEwADgU#v=onepage&q=linkers%20and%20adaptors%20in%20molecular%20biology&f=false

http://books.google.com.my/books?id=OWXM4Q3- eEC&pg=PA72&lpg=PA72&dq=linker+and+adaptor+for+cloning&source=bl&ots=xrsfLnbNUZ&sig=HO3B_pAJ3CRXWDOd-n0Iov5cks&hl=en&sa=X&ei=RTeEVNH5B8OcugTjvIGYBA&ved=0CCAQ6AEwATgK#v=onepage&q=linker%20and%20adaptor%20for%20cloning&f=false

http://bsp.med.harvard.edu/?q=node/18

http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%20101%20laboratory/bacterial%20transformation/bacteria.htm

http://www.addgene.org/plasmid-protocols/bacterial-transformation/

http://www.bio-rad.com/en-ml/applications-technologies/gene-cloning-analysis  

http://www.chemheritage.org/discover/online-resources/chemistry-in-history/themes/pharmaceuticals/preserving-health-with-biotechnology/berg-boyer-cohen.aspx

http://www.idtdna.com/pages/decoded/decoded-articles/core-concepts/decoded/2012/06/15/cloning-strategies-part-3-blunt-end-cloning

http://www.jove.com/science-education/5069/dna-ligation-reactions

http://www.lifetechnologies.com/my/en/home/life-science/cloning/competent-cells-for-transformation/chemically-competent.html

http://www.researchgate.net/profile/Kishwar_Khan2/publication/257396774_Vectors_used_in_gene_manipulation-a_retrospective/links/0046352544362d7f23000000

http://www.sigmaaldrich.com/china-mainland/zh/technical- documents/articles/biology/competent-cell-selection-guide.html

http://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/biology/blue-white-screening.html

http://www2.le.ac.uk/departments/genetics/vgec/highereducation/topics/recombinanthttp://www.wisegeek.org/what-is-recombinant-dna.htm

https://explorable.com/transforming-principle

https://www.youtube.com/watch?v=51J_pHkPLgw

https://www.youtube.com/watch?v=MeMddsYl_TE    linker n adaptor

https://www.youtube.com/watch?v=rhd_fBPyzSM

https://www.youtube.com/watch?v=TUWif0porA4 dna ligase

https://www.youtube.com/watch?v=xD-1VVG4Wf0

https://www.youtube.com/watch?v=ZW9zPdb_Bs0

www.addgene.org/plasmid-protocols/bacterial-transformation/       

www.csun.edu/~hcbio027/biotechnology/lec2/RE/re.htm

www.discoverbiotech.com/wiki/-/wiki/Main/Applications+of+Cloning    

www.mfa.od.ua/page144.html

 

 

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