GENE CLONING
Principle
Gene cloning
Gene cloning is the production of exact copies of DNA sequences. Restriction enzyme is used to split the fragment of DNA contain target gene. The fragments are inserted into cloning vectors. Then, the recombinant DNA will transfer to suitable host and undergo replication. Various screening method is used to identify desired clones.
Transformation
Transformation is a process which foreign DNA is introduced and uptake by a cell to make large quantities of it. Plasmid is used to amplify gene of interest. Plasmid consists of selectable marker, origin of replication and multiple cloning sites are suitable or transformation. Meanwhile, competent cell is make bacteria more easier to uptake plasmid since DNA is difficult pass through cell membrane of bacteria.
Recombinant DNA (rDNA)
Recombinant DNA is a DNA inserted into different set of DNA. It is used in genetic manipulation to create new organisms. Recombinant DNA is introduced into a vector. Microinjection and biolistics are methods to introduce rDNA. Recombinant DNA is used to add specific features into crops, bacteria and animal in order to bring benefit to humans.
