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GENE CLONING

Summary : Cloning
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Choose the suitable DNA sample and vector for cloning process.
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Cut both of the sample DNA and vector DNA by using same restriction enzyme (EcoRI) at restriction recognition site.
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Incubate both restricted sample DNA and vector DNA together.
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Recombination occur at the DNA sticky ends due to the presence of enzyme, ligase.
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Insert the recombinant DNA into the bacterial host cell. This process known as transformation.
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The recombinant DNA is allowed to replicate as the host cell replicate.
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