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Summary : Cloning

 

  1. Choose the suitable DNA sample and vector for cloning process.

  2. Cut both of the sample DNA and vector DNA by using same restriction enzyme (EcoRI) at restriction recognition site.

  3. Incubate both restricted sample DNA and vector DNA together.

  4. Recombination occur at the DNA sticky ends due to the presence of enzyme, ligase.

  5. Insert the recombinant DNA into the bacterial host cell. This process known as transformation.

  6. The recombinant DNA is allowed to replicate as the host cell replicate.

 

 

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